Zyppy-96 Plasmid Kit (4 x 96 Preps)

The Zyppy-96 Plasmid Miniprep is the fastest and simplest high-throughput method available for efficient isolation of plasmid DNA from E. coli. The kit features a modified, Pellet-Free alkaline lysis system that bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to the bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are expedited using a specially designed Neutralization/Clearing Buffer. The remaining DNA purification steps are straightforward and simple. Eluted plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection.